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2 edition of Transcription activation by the cyclic AMP receptor protein found in the catalog.

Transcription activation by the cyclic AMP receptor protein

David Mackay West

Transcription activation by the cyclic AMP receptor protein

by David Mackay West

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  • 20 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D) - University of Birmingham, School of Biochemistry, Faculty of Science, 1995.

Statementby David Mackay West.
ID Numbers
Open LibraryOL17203986M

COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus. Transcription Activation Mediated by a Cyclic AMP Receptor Protein from Thermus thermophilus HB8 Article (PDF Available) in Journal of Bacteriology (10) June with 69 Reads.

One transcription factor is cyclic AMP (cAMP) receptor protein (Crp), a soluble receptor for the signaling molecule 3′,5′-cAMP found in diverse prokaryotic and eukaryotic organisms (6, 21). Crp responds to intracellular cAMP transported into the cell from the exterior milieu or synthesized by an endogenous adenylate cyclase (11). Introduction. The cyclic AMP receptor protein (CRP, also known as the catabolite activator protein, CAP) is a global transcription factor, which plays a central role in the control of metabolism in Escherichia coli and other enteric bacteria (Kolb et al., ; Barrett et al., ).CRP, which is functional as a homodimer, recognizes 22 bp target sequences, with the consensus 5.

Activates transcription. Positively regulates six promoters upstream of the TTHB, TTHB, TTHB, TTHB, TTHA and TTHA genes in a cAMP-dependent manner. Regulated genes include clustered regularly interspaced short palindromic repeat (CRISPR) associated (Cas) genes, and the genes encoding a putative transcriptional regulator, a protein containing the exonuclease III-like . Axonal injury induces expression of the svh-2 gene encoding a receptor tyrosine kinase, stimulation of which by the SVH-1 growth factor leads to activation of the JNK pathway. Here, we identify ETS-4 and CEBP-1, related to mammalian Ets and C/EBP, respectively, as transcriptional activators of svh-2 expression following axon injury.


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Transcription activation by the cyclic AMP receptor protein by David Mackay West Download PDF EPUB FB2

Introduction. Cyclic AMP (cAMP) receptor proteins (CRPs) are global transcriptional regulators that are widely distributed in bacteria[1, 2].They play important roles in the regulation of many biological processes, including adaptation to starvation and extreme temperatures, energy metabolism, cell division, and toxin production[3–7].As a transcription factor, the CRP/FNR family has diverse Cited by: Cyclic AMP (cAMP) receptor proteins (CRPs) are global transcriptional regulators broadly distributed in bacteria (30, 72).The cellular roles of such CRP family proteins are diverse and include carbohydrate metabolism (3, 30), development of competence for transformation (), modulation of virulence gene expression and pathogenesis (10, 11, 55, 57, 65), resuscitation (), and germination and Cited by: The Escherichia coli rhaSR operon encodes two AraC family transcription activator proteins, RhaS and RhaR, which regulate expression of the l-rhamnose catabolic regulon in response to l-rhamnose positively regulates rhaSR in response to l-rhamnose, and RhaR activation can be enhanced by the cyclic AMP (cAMP) receptor protein (CRP) by: Mutations in either the adenylate cyclase gene (cya) or the crp gene encoding the cyclic AMP receptor protein result in expression of toxin and pilus under repressing growth conditions [].

CRP is a transcription factor that, when complexed with cAMP, binds DNA and activates transcription of many genes in response to low glucose levels. 1. Introduction. The cyclic AMP receptor protein (CRP, also known as CAP) of Escherichia coli is a transcription factor that regulates the expression of more than genes in the bacterium.It is a homodimer of residue subunits, comprising the C-terminal DNA binding domain (DBD, residues –) and the N-terminal dimerisation domain that binds cAMP (CBD, Cited by: 6.

The structure of this pleiotropic activator of gene transcription in bacteria and its interaction sites at promoter DNA's as well as the role of this protein in the RNA polymerase-promoter interactions are reviewed.

Cyclic AMP receptor protein: role in transcription activation. B de Cyclic AMP receptor protein: role in transcription Cited by: Abstract. Cyclic AMP (cAMP) serves a central role in controlling the catabolic activity of both prokaryotic and eukaryotic cells.

In bacteria the effects of cAMP are mediated by the cAMP receptor protein. Cyclic adenosine monophosphate (cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate) is a second messenger important in many biological processes.

cAMP is a derivative of adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying the cAMP-dependent should not be confused with 5'-AMP-activated protein kinase (AMP.

de Crombrugghe B, Busby S, Buc H. Cyclic AMP receptor protein: role in transcription activation. Science. May 25; ()– Garner MM, Revzin A. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory by: The activated Escherichia coli cAMP receptor protein, CRP, is capable of regulating the expression of more than 20 genes by binding to specific DNA sites.

DNA bending is an important structural feature that has been observed in the regulatory mechanism of gene expression by CRP. On the basis of the results of the fluorescence energy transfer study of the gal P1 promoter, gal bends. Transcription activation by the Escherichia coli cyclic AMP receptor protein (CRP) at Class II promoters is dependent on direct interactions between two surface-exposed activating regions (AR1 and AR2) and two contact sites in RNA polymerase.

Many of the effects of prototypical second messenger cyclic adenosine 3′,5′-monophosphate (cAMP) on complex processes such as the regulation of fuel metabolism, spermatogenesis and steroidogenesis are mediated via changes in target gene transcription.

A large body of research has defined members of the cAMP-response element binding (CREB) protein family. CREB-TF (CREB, cAMP response element-binding protein) is a cellular transcription binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the genes.

CREB was first described in as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c. Cyclic AMP response element-binding protein (CREB) activates genes that contain regulatory cre-elements, such as the immediate-early gene c-fos, and both CREB and the activated genes are important for NCAM-mediated neurite outgrowth.

CREB is activated by the MAPK pathway, leading to its phosphorylation and activation of transcription. AMPare mediated by the cyclic AMP receptorprotein (CRPorCAP),whereas in eukaryotes they are mediated by a cyclic AMP-dependent protein kinase.

In both types of cells the role of these proteins is to sensetheintracellularcon-centration of cyclic AMP. In bacteria these levels vary primarily with the car-bohydrate substrate on which the cells grow(1).

The best studied of this type of activator is the cyclic AMP receptor protein (CRP) (also referred to as catabolite gene activator protein (CAP)). In the lac system, CRP binds as a dimer to a site upstream of the lac promoter, and the subunit that is proximal to the promoter contacts the C-terminal domain (αCTD) of one of the α subunits of.

In Escherichia coli, cyclic AMP receptor protein (CRP) can regulate the transcription of more than genes.

The signal to activate CRP is the binding of cyclic AMP. Binding of cAMP to CRP leads to a long-distance signal transduction from the N-terminal cAMP-binding domain to the C-terminal domain of the protein, which is responsible for.

Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein.

Savery N, Rhodius V, Busby S Philos Trans R Soc Lond B Biol Sci, (), 01 Apr Objective: This study investigated the role and mechanism of action of G protein-coupled estrogen receptor (GPER) in melanogenesis.

Methods: GPER expression was detected in the A human melanoma cell line and B16 mouse melanoma cell line. Cell proliferation, melanin content, tyrosinase (TYR) activity, cyclic adenosine monophosphate (cAMP) level, and TYR and microphthalmia-related.

Alpha2-adrenergic receptors activate cyclic AMP-response element-binding protein through arachidonic acid metabolism and protein kinase A in a subtype-specific manner J Neurochem. Nov;(3) doi: /jx. History. Protein kinase A, more precisely known as adenosine 3',5'-monophosphate (cyclic AMP)-dependent protein kinase was discovered by chemists Edmond H.

Fischer and Edwin G. Krebs in They won the Nobel Prize in Physiology or Medicine in for their work on phosphorylation and dephosphorylation and how it relates to protein kinase A activity.The activity of c-Jun, the major component of the transcription factor AP-1, is potentiated by amino-terminal phosphorylation on serines 63 and 73 (Ser/73).

This phosphorylation is mediated by the Jun amino-terminal kinase (JNK) and required to recruit the transcriptional coactivator CREB-binding .Under fasting conditions, activation of several hepatic genes sets the stage for gluconeogenesis in the liver. cAMP response element-binding protein (CREB), CREB-regulated transcription coactivator 2 (CRTC2), and peroxisome proliferator-activated receptor γ coactivator 1-alpha (PGC-1α) are essential for this transcriptional induction of gluconeogenic genes.